Untersucht wurden die Gewebeproben von Primärtumoren und Lymphknotenmetastasen von 25 Patienten mit zuvor unbehandelten Plattenepithelkarzinomen des Oropharynx.
Als Methode diente der immunhistochemische Nachweis von ki-67, CD-105, HIF-1α, Carbonic Anhydrase 9 (CA 9) und Thymidin Phosphorylase (TP) mit Hilfe von spezifischen Antikörpern.
Die Auswertung erfolgte Computer assistiert für CD-105 und ki-67 Färbungen.
www.egms.de47 Patients attending the ENT-Department University Hospital of RWTH Aachen with previously untreated squamous cell carcinoma of the oropharynx were included in this study.
Tissue samples of tumor and tumor surrounding tissue were collected in tissue microarrays and stained with specific antibodies against hypoxia-inducible-factor-1 (HIF-1), carbonic anhydrase 9 (CA 9), thymidine phosphorylase TP and prolyl hydoxylase 2 (PHD-2).
The amount of staining was classified in four grades by two independent investigators.
www.egms.de47 Patients attending the ENT-Department University Hospital of RWTH Aachen with previously untreated squamous cell carcinoma of the oropharynx were included in this study.
Tissue samples of tumor and tumor surrounding tissue were collected in tissue microarrays and stained with specific antibodies against hypoxia-inducible-factor-1 (HIF-1), carbonic anhydrase 9 (CA 9), thymidine phosphorylase TP and prolyl hydoxylase 2 (PHD-2).
The amount of staining was classified in four grades by two independent investigators.
www.egms.deUntersucht wurden die Gewebeproben von Primärtumoren und Lymphknotenmetastasen von 25 Patienten mit zuvor unbehandelten Plattenepithelkarzinomen des Oropharynx.
Als Methode diente der immunhistochemische Nachweis von ki-67, CD-105, HIF-1α, Carbonic Anhydrase 9 (CA 9) und Thymidin Phosphorylase (TP) mit Hilfe von spezifischen Antikörpern.
Die Auswertung erfolgte Computer assistiert für CD-105 und ki-67 Färbungen.
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